Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo INRA Logo ISO 9001:2008 certified Logo RARe Logo Agrocampus Ouest - Institute for life, food and horticultural sciences and landscaping  Logo AgroParisTech - Paris institute of technology for life, food and environmental sciences Logo Aix Marseille University Logo Angers University

Home page

Reviving freezed-dryed bacteria

The revival of the strains must be done by qualified staff, in an appropriate laboratory with equipment providing aseptic conditions.

Material:

  • A diamond tool
  • Pasteur pipettes
  • A Pasteur pipette with round end or a loop

Media:

  • 5 ml of peptone nitrated water or other nutrient broth
  • Nutrient agar plates, adapted to the strains

Reviving freezed-dried bacteria:

Strains revival in video:

Make stripes all arround the narrow part of the vial with the diamond tool. 
Sterilize the narrow part of the vial. 
Break the vial in aseptic conditions

Resuspend the lyophilisat with 300µl of the nutrient broth by multiple pipeting. Ensure that the lyophilisat is well resuspended.
Two drops are laid down on the agar plate, the residual bacterial suspension is added to the broth.
One drop is used to spread the inoculum in order to obtain isolated colonies after incubation. 
The other is kept untouched in order to preserve a concentrated inoculum.

Agar plates and tubes are incubated at the optimal temperature for growth.

If no growth occurs on the plates after recommended incubation time, it is possible to spread the nutrient broth on new nutrient agar plates.

CIRM-CFBP_DessinOvertureAmpoule

Composition of usual media:

peptone nitrated water pH 7.2

Bacto peptone

10g

NaCl
Distilled water

5g
1000ml

YPGA medium pH 7,2

Y: Yeast extract (Mérieux)
P: Bacto peptone (Mérieux)

7g
7g

G : Glucose
A : Agar Agar

7g
15g

Distilled water

1000ml