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INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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Services

The CIRM-BP proposes to carry out the provision of services to meet your needs in the areas of identification, phenotypic and molecular characterization and conservation of strains. The CIRM-BP also proposes to characterize the antibacterial potential of your molecules on a panel of pathogenic bacteria. 

Molecular indentification and taxonomic classification of the bacterial strains at the species level

The molecular identification can be performed by sequencing of the 16S rDNA or other more appropriate genes if necessary.

Strains molecular typing

Molecular typing can be achieved by MLVA (Multi Locus VNTR (Variable Number of Tandem Repeats) Analysis) or MLST (Multi Locus Sequence Typing) depending on the existing techniques and on the context and purpose of the typing. The development of these techniques can also be considered if necessary.

Determination of the strains virulence genes repertoire

Strains are subjected to PCR for the study of the virulence genes repertoire based on what is known for the considered bacterial species.

Preparation of genomic DNA

Depending on demand, the control of the extracted DNA may include the following steps:

  • Gel migration
  • Nanodrop quantification
  • PCR amplification
  • Molecular authentication

Identification and phenotypic characterization of strains of pathogenic bacteria

The conventional bacteriological techniques are used to determine the phenotypic profile of the strains and to identify them. Moreover, the growth parameters of strains can be compared in different culture conditions (temperature, medium, pH) using the Bioscreen C (turbidimetric method).

Antibiotic Sensitivity Testing

Antibiotic sensitivity testing (AST) aims to determine the susceptibility of an isolate to a range of potential therapeutic agents. This can be done either by disk diffusion method or Minimum Inhibitory Concentration (MIC) determination by broth dilution method in microtitre-plates.

Confidential Safe Deposits

The strains are both cryopreserved in liquid nitrogen and by freeze-drying. For security reasons, strains are identified at the species level prior to conservation. The responsability of the strains origin and their provenance is entirely assumed by the applicant who will retain ownership and exclusive use of the deposited strains. More...

Characterization of the antimicrobial potential of molecules of interest on a panel of pathogenic bacteria

The antimicrobial activity spectrum may be evaluated with several methods like the spectrophotometric determination of bacterial growth kinetics in the presence of the test molecule (use of microplates and Bioscreen C apparatus) and the determination of diameters of inhibition by radial diffusion in agar...