Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo INRA Logo ISO 9001:2008 certified Logo RARe Logo Agrocampus Ouest - Institute for life, food and horticultural sciences and landscaping  Logo AgroParisTech - Paris institute of technology for life, food and environmental sciences Logo Aix Marseille University Logo Angers University

Home page

Services

Molecular taxonomical identification of yeasts to the species level

The molecular identification is obtained by:

  • Amplification and sequencing of the D1/D2 domain of 26S rRNA gene
  • Sequence comparison to the sequences in the publicly available databases (Kurtman & Robnett 1997, J Clin. Microbiol. 38, 1216-1223; Kurtman & Robnett 1998, Antonie Van Leeuwenhoek. 73, 331-371).

The molecular identification by sequence analysis of the D1/D2 domain is carried out within approximately 10 days after reception of the strain. Results of analyses are sent by electronic mail and by post.

Phenotypic characterisation of yeasts

The following markers are checked according to the procedure described by J.P. van der Walt and D. Yarrow (1984, The Yeasts a taxonomic study, N.J.W. Kreger-van Rij ed.):

  • Fermentation of ten sugars
  • Assimilation of 32 carbon source (ID32 gallery)
  • Assimilation of eight nitrogen source
  • Growth on up to 0.01% cycloheximide
  • Growth on 50% glucose
  • Growth at four different temperatures: 30 °C, 35°C, 37°C and 40°C
  • Production of urease on Christensen medium
  • Arbutin degradation
  • Starch degradation (for certain species)
  • Sporulation

The phenotypic characterization is carried out within approximately three weeks after reception of the strain. Results of analyses are sent by electronic mail and by post.

Confidential Safe Deposit Service

Strains are cryo-preserved. We are required for safety of the staff to identify the strains to the species level before their conservation. Designation of their origin and their source is the responsibility of the depositor. Deposit will be effective one month after the reception of the  strain. A strain deposited at the CIRM-Levures will remain the property of the depositor and will not appear in the strain catalogue. A culture of the strain will be provided to the depositor on request within one week. This service will be charged (to see the deposit contract, click here).

Confidential Safe Deposit Service associated to molecular sub-species identity

Confidential safe deposit can be associated with the molecular sub-species identity of yeast stocks. Designation of their origin and their source is the responsibility of the depositor. Deposit will be effective one month after the reception of the  strain. A strain deposited at the CIRM-Levures will remain the property of the depositor and will not appear in the strain catalogue. A culture of the strain will be provided  to the depositor on request within one week. This service will be charged. Techniques used are:

  • Classical taxonomy identification
  • Molecular taxonomy identification
  • Electrophoretic karyotyping
  • Mitochondrial DNA RFLP
  • Inter-delta PCR typing
  • Any other reliable method of sub-species taxonomical characterization decided in agreement with the depositor, whenever possible

Strain molecular characterization using the following techniques (when applicable)

  • Electrophoretic karyotyping
  • Mitochondrial DNA RFLP
  • Inter-delta PCR fingerprinting (applicable to species for which complete genome sequence has been determined, see Sohier et al (2009) Dairy Sci.Technol. 89:569-581 (PDF)
  • Determination of ploidy using flow cytometry as described in Jacques et al (2010) Eukaryot Cell. 9:449-59. (PDF).

The time necessary for strain charaterization will depend on the number of strains and the type of method applied.

Construction of hybrids

Cells (or spores after ascus dissection, if necessary) are brought together using micromanipulation and the resulting zygotes are analyzed by flow cytometry, elecrophoretic karyotyping and marker analysis. Stability of the genome hybrids is monitored over 100 generations. The time necessary for the hybrid construction will depend on the project.

Determination of yeast in foodstuffs

Dilutions of re-suspended foodstuffs in water are spread onto YPD supplemented with 200µg/ml of streptomycin. The resulting colonies are counted. The determination of yeast will be performed in about two weeks after the reception of the foodstuff.

Viability of Active Dry Yeasts

After re-suspension of Active Dry Yeasts in water, cell number is determined using a Malassez cell, and cell growth is determined by colony counting on Petri dishes. The analysis of the viability of Active Dry Yeasts will be performed in about two weeks after their reception.