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Publications & Outcomes

POSITIVe publications and other outcomes on research conducted through partnerships will be listed below.

Action Publications

Addressing the inter-individual variation in response to consumption of plant food bioactives - towards a better understanding of their role in healthy ageing and cardiometabolic risk reduction. Manach C, Milenkovic D, Van de Wiele T, Rodriguez-Mateos A, de Roos B, Garcia-Conesa MT, Landberg R, Gibney ER, Heinonen M, Tomás-Barberán F, Morand C. Mol Nutr Food Res. 2016 Sep 30. doi: 10.1002/mnfr.201600557. [Epub ahead of print] Review. PubMed

Fig Manach et al

Impact of Flavanols on Cardiometabolic Biomarkers: a meta-analysis of randomized controlled human trials to explore the role of inter-individual variability. Menezes R, Rodriguez-Mateos A, Kalsatou A, Gonzalez-Sarrias A, Greyling A, Giannaki C, Andres-Lacueva C, Milenkovic D, Gibney E.R, Dumont J, Schär M, Garcia-Aloy M, Palma-Duran S A, Ruskovska T, Maksimova V, Combet E, Pinto P. Nutrients2017, 9(2), 117; doi:10.3390/nu9020117

Host-related factors explaining interindividual variability of carotenoid bioavailability and tissue concentrations in humans. T. Bohn, C. Desmarchelier, L.O. Dragsted, C.S. Nielsen, W. Stahl, R. Rühl, J. Keijer, P. Borel. Mol Nutr Food Res. 2017 Jan 19. doi: 10.1002/mnfr.201600685

MNFR 2017 Graphical Abstract

Interindividual Variability in Biomarkers of Cardiometabolic Health after Consumption of Major Plant-Food Bioactive Compounds and the Determinants Involved. Milenkovic D, Morand C, Cassidy A, Konic-Ristic A, Tomás-Barberán F, Ordovas JM, Kroon P, De Caterina R, Rodriguez-Mateos A. Adv Nutr. 2017 Jul 14;8(4):558-570. doi: 10.3945/an.116.013623. Open Access

A Systematic Review and Meta-Analysis of the Effects of Flavanol-Containing Tea, Cocoa and Apple Products on Body Composition and Blood Lipids: Exploring the Factors Responsible for Variability in Their Efficacy.  Antonio González-Sarrías, Emilie Combet, Paula Pinto, Pedro Mena, Margherita Dall’Asta, Mar Garcia-Aloy, Ana Rodríguez-Mateos, Eileen R. Gibney, Julie Dumont, Marika Massaro, Julio Sánchez-Meca, Christine Morand and María-Teresa García-Cones. Nutrients2017 Open Access


Bioavailability of Quercetin in Humans with a focus on inteindividual variation. A.Filipa Almeida, Grethe Iren A. Borge, Mariusz Piskula, Adriana Tudose, Liliana Tudoreanu, Katerine Valentova, Gary Williamson, Claudia N. Santos. Comprehensice Reviews in Food Science and Food Safety. 2018  Open Access


Meta-Analysis of the Effects of Foods and Derived Products Containing Ellagitannins and Anthocyanins on Cardiometabolic Biomarkers: Analysis of Factors Influencing Variability of the Individual Responses. María-Teresa García-Conesa,Karen Chambers,Emilie Combet,Paula Pinto,Mar Garcia-Aloy,Cristina Andrés-Lacueva,Sonia de Pascual-Teresa,Pedro Mena,Aleksandra Konic Ristic,Wendy J. Hollands,Paul A. Kroon,Ana Rodríguez-Mateos,Geoffrey Istas,Christos A. Kontogiorgis,Dilip K. Rai,Eileen R. Gibney,Christine Morand,Juan Carlos Espín and Antonio González-Sarrías. Int. J. Mol. Sci. 201819(3), 694; Open Access

Graphical Abstract_Garcia-Conesa et al Int J Mol Sci 2018

Other Outcomes

The metabolic pathway of flavan-3-ols (also known as flavanols) in humans. Eirini Deligiannidou, Egon Willighagen, Denise Slenter.

Description: Flavanols are derivatives of flavans and include catechin, epicatechin gallate, epigallocatechin, epigallocatechin gallate, proanthocyanidins, theaflavins, thearubigins. These compounds differ considerably not only in their structural and functional properties, but also in their metabolism and bioavailability. Green arrows indicate microbiota-mediated steps, and Red arrows represent mammalian enzyme-mediated conversions. Pink arrows indicate steps that can be both microbiota- or enzyme-mediated. This pathway was developed based upon 22 papers, therefore black arrows indicate missing information regarding the nature of conversion.

Link to Wikipathways

Publication with COST POSITIVe acknowledgment

WG1: Inter-individual variation in bioavailability

Targeted and Untargeted Metabolomics to Explore the Bioavailability of the Secoiridoids from a Seed/Fruit Extract (Fraxinus angustifolia Vahl) in Human Healthy Volunteers: A Preliminary Study. Rocío García-Villalba, Francisco A. Tomás-Barberán, Pascale Fança-Berthon, Marc Roller, Pilar Zafrilla, Nicolas Issaly and María-Teresa García-Conesa. Molecules2015, 20, 22202–22219. Abstract

Influence of age on the absorption, metabolism, and excretion of cocoa flavanols in healthy subjects. Ana Rodriguez-Mateos, Tania Cifuentes-Gomez, Isidro Gonzalez-Salvador, Javier I. Ottaviani, Hagen Schroeter, Malte Kelm, Christian Heiss and Jeremy P. E. Spencer. Mol. Nutr. Food Res.2015; DOI: 10.1002/mnfr.201500091 Abstract

Plasma and Urinary Phenolic Profiles after Acute and Repetitive Intake of Wild Blueberry. Rodrigo P. Feliciano, Geoffrey Istas, Christian Heiss and Ana Rodriguez-Mateos. Molecules2016, 21, 1120. doi: 10.3390/molecules21091120 Abstract

Bioavailability of wild blueberry (poly)phenols at different levels of intake. Rodriguez-Mateos A, Feliciano RP, Cifuentes-Gomez T and Spencer JPE. J Berry Res2016; 6(2), 137-148. doi: 10.3233/JBR-160123 Abstract

Development and validation of a high-throughput micro solid-phase extraction method coupled with ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry for rapid identification and quantification of phenolic metabolites in human plasma and urine. Feliciano RP, Mecha E, Bronze MR, Rodriguez-Mateos A. J Chromatogr A. 2016 Sep 16; 1464:21-31. doi: 10.1016/j.chroma.2016.08.027 Abstract

Identification and quantification of novel cranberry-derived plasma and urinary (poly)phenols. Feliciano RP, Boeres A, Massacessi L, Istas G, Ventura MR, Nunes Dos Santos C, Heiss C, Rodriguez-Mateos A. Arch Biochem Biophys. 2016; 599:31-41.  doi: 10.1016/ Abstract

Urolithins, the rescue of 'old' metabolites to understand a 'new' concept: metabotypes as a nexus between phenolic metabolism, microbiota dysbiosis and host health status. Tomás-Barberán FA, González-Sarrías A, García-Villalba R, Núñez-Sánchez MA, Selma MV, García-Conesa MT, Espín JC. Mol Nutr Food Res. 2016 May 9. doi: 10.1002/mnfr.201500901. Abstract

Resveratrol metabolite profiling in clinical nutrition research--from diet to uncovering disease risk biomarkers: epidemiological evidence. Rabassa M, Zamora-Ros R, Urpi-Sarda M, Andres-Lacueva C. Ann N Y Acad Sci.2015; 1348(1):107-15. doi: 10.1111/nyas.12851 Abstract

The role of metabolism (and the microbiome) in defining the clinical efficacy of dietary flavonoids, Cassidy A and Minihane  AM, Am J Clin Nutr, in press, Abstract

WG2: Inter-individual variation in the biological responsiveness regarding cardiometabolic endpoints

Back to the future: Epigenetic clock plasticity towards healthy aging. Declerck K1, Vanden Berghe W2.Mech Ageing Dev. 2018 Jan 11. pii: S0047-6374(17)30247-6. doi: 10.1016/j.mad.2018.01.002. [Epub ahead of print]

Critical Evaluation of Gene Expression Changes in Human Tissues in Response to Supplementation with Dietary Bioactive Compounds: Moving Towards Better-Quality Studies. Pokimica B, García-Conesa MT. Nutrients. 2018 Jun 22;10(7). pii: E807. doi: 10.3390/nu10070807. Review.

Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid Hormone availability in acyclic female rats. Šošić-Jurjević B, Lütjohann D, Jarić I, Miler M, Vojnović Milutinović D, Filipović B, Ajdžanović V, Renko K, Wirth EK, Janković S, Kӧhrle J, Milošević V.  Exp Gerontol. 2017 Jun;92:74-81. Abstract

Role of sulfotransferases in resveratrol metabolism in human adipocytes. N. Gheldof, S. Moco, C. Chabert, T. Teav, D. Barron, and J. Hager (2017) , Molecular Nutrition and Food Research, 61 (10). Abstract

Cocoa flavanol intake improves endothelial function and Framingham Risk Score in healthy men and women: a randomised, controlled, double-masked trial: the Flaviola Health Study. Roberto Sansone, Ana Rodriguez-Mateos, Jan Heuel, David Falk, Dominik Schuler, Rabea Wagstaff, Gunter G. C. Kuhnle, Jeremy P. E. Spencer, Hagen Schroeter, Marc W. Merx, Malte Kelm, Christian Heiss and for the Flaviola Consortium, European Union 7th Framework Program. British Journal of Nutrition 2016 114(08):1246-1255. Abstract

Epigenetic Control of Cardiovascular Health by Nutritional Polyphenols Involves Multiple Chromatin-Modifying Writer-Reader-Eraser Proteins. K. Declerck, K. Szarc vel Szic, A. Palagani, K. Heyninc, G. Haegeman,Ch. Morand, D. Milenkovic and W. Vanden Berghe. Current Topics in Medicinal Chemistry 2016. Abstract

From inflammaging to healthy aging by dietary lifestyle choices: is epigenetics the key to personalized nutrition? K. Szarc vel Szic, K. Declerck, M. Vidakovic, W. Vanden Berghe. Clinical Epigenetics 2015, 7:33. DOI:10.1186/s13148-015-0068-2 Abstract

Flavonoid intake and cardiovascular disease risk. Rodrigo P Feliciano, Sarah Pritzel, Christian Heiss and Ana Rodriguez-Mateos. Current Opinion in Food Science 2015, 2:92–99. DOI:10.1016/j.cofs.2015.02.006 Abstract

Impact of cocoa flavanol intake on age-dependent vascular stiffness in healthy men: a randomized, controlled, double-masked trial. Christian Heiss, Roberto Sansone, Hakima Karimi, Moritz Krabbe, Dominik Schuler, Ana Rodriguez-Mateos, Thomas Kraemer, Miriam Margherita Cortese-Krott, Gunter G. C. Kuhnle, Jeremy P. E. Spencer, Hagen Schroeter, Marc W. Merx,  Malte Kelm. AGE 2015,37:56. DOI:10.1007/s11357-015-9794-9 Abstract

Direct comparison of metabolic health effects of the flavonoids quercetin, hesperetin, epicatechin, apigenin and anthocyanins in high-fat-diet-fed mice. Elise F. Hoek-van den Hil,  Evert M. van Schothorst,  Inge van der Stelt, Hans J. M. Swarts,  Marjanne van Vliet, Tom Amolo,  Jacques J. M. Vervoort, Dini Venema, Peter C. H. Hollman, Ivonne M. C. M. Rietjens,  Jaap Keijer. Genes Nutr 2015,10:23. DOI:10.1007/s12263-015-0469-z Abstract

Citrus flavanones naringenin and hesperetin improve antioxidant status and membrane lipid compositions in the liver of old-aged Wistar rats. Marko Miler, Jasmina Živanović, Vladimir Ajdžanović, Zorana Oreščanin-Dušić, Dragan Milenković, Aleksandra Konić-Ristić, Duško Blagojević, Verica Milošević, Branka Šošić-Jurjević. Experimental Gerontology 84 (2016) 49–60. doi: 10.1016/j.exger.2016.08.014 Abstract

Functional morphology of pituitary -thyroid and -adrenocortical axes in middle-aged male rats treated with Vitex agnus castus essential oil. BrankaˇSoˇsi´c-Jurjevi´, Vladimir Ajdˇzanovi´, Branko Filipovi´, Svetlana Trifunovi´, Ivana Jari´, Nataˇsa Risti´, Verica Miloˇsevi´. Acta Histochemica (2016), DOI: 10.1016/j.acthis.2016.07.007. 

An update on the role of nutrigenomic modulations in mediating the cardiovascular protective effect of fruit polyphenols. I. Krga, D. Milenkovic, C. Morand and L.-E. Monfoulet. Food Funct.,2016, DOI: 10.1039/c6fo00596a.  doi: 10.1039/c6fo00596a Abstract

Histological study of the ovaries from the superovulated mouse supplemented by bioactive feed additive. Vanya Mladenova, Desislava Abadjieva, Elena Kistanova. Journal of Mountain Agriculture on the Balkans, vol. 19, 2, 2016, (23-36).

Cranberry (poly)phenol metabolites correlate with improvements in vascular function: A double-blind, randomized, controlled, dose-response, crossover study.Rodriguez-Mateos A, Feliciano RP, Boeres A, Weber T, Dos Santos CN, Ventura MR, Heiss C. Mol Nutr Food Res. 2016; 60(10):2130-2140. doi: 10.1002/mnfr.201600250 Abstract

WG 3: From emerging science to applications

Spanish Consumers’ General Understanding of Metabolic Diseases and how they may be Influenced by Diet and Novel Foods: a Preliminary Survey. Laura Laguna, Begoña Bartolomé, M. Victoria Moreno-Arribas, M. Teresa García-Conesa. Research Article – Nutr Res Food Sci J. DOI: 10.31038/NRFSJ.1000102

Analytical Methods - Molybdenum disulphide and graphene quantum dots as electrode modifiers for laccase biosensor. Ioana Vasilescu, Sandra A.V. Eremia*, Mihaela Kusko, Antonio Radoi*, Eugeniu Vasile, Gabriel-Lucian Radu.   Biosensors and Bioelectronics, Volume 75, 15 January 2016, Pages 232-237. Abstract

Other recent articles published by POSITIVe partners with POSITIVe acknowledgement.

Figueira, I; Garcia, G; Pimpão, RC; Terrasso;AP; Costa, I.; Almeida, AF; Tavares; L; Pais, TF; Pinto, P; Ventura, MR; Filipe, A; McDougall, G; Stewart, D; Kim, KS; Palmela, I; Brites, D; Brito, MA; Brito, C; Santos, CN, Polyphenols journey through blood-brain barrier towards neuronal protection, Scientific Reports, doi:10.1038/s41598-017-11512-6.

Bioactivity of Polyphenols: Preventive and Adjuvant Strategies toward Reducing Inflammatory Bowel Diseases-Promises, Perspectives, and Pitfalls. Kaulmann A, Bohn T. Oxid Med Cell Longev. 2016;2016:9346470. doi: 10.1155/2016/9346470

Bioactivity of Carotenoids - Chasms of Knowledge. Bohn T. Int J Vitam Nutr Res. 2017 Feb 10:1-5. doi: 10.1024/0300-9831/a000400

Purchartová, K.; Valentová, K.; Pelantová, H.; Marhol, P.; Cvačka, J.; Havlíček, L.; Křenková, A.; Vavříkova, E.; Biedermann, D.; Chambers, C. S.; Křen, V., Prokaryotic and Eukaryotic Aryl Sulfotransferases: Sulfation of Quercetin and Its Derivatives. Chemcatchem, 2015, 7 (19), 3152-3162. DOI 10.1002/cctc.201500298

Vavrikova, E.; Langschwager, F.; Jezova-Kalachova, L.; Krenkova, A.; Mikulova, B.; Kuzma, M.; Kren, V.; Valentova, K., Isoquercitrin Esters with Mono- or Dicarboxylic Acids: Enzymatic Preparation and Properties. International Journal of Molecular Sciences, 2016, 17 (6).  DOI 10.3390/ijms17060899

Karas, D.; Ulrichova, J.; Valentova, K., Galloylation of polyphenols alters their biological activity. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association 2017, 105, 223-240. DOI 10.1016/j.fct.2017.04.021

Heřmánková-Vavříková, E.; Křenková, A.; Petrásková, L.; Chambers, C.; Zápal, J.; Kuzma, M.; Valentová, K.; Křen, V., Synthesis and Antiradical Activity of Isoquercitrin Esters with Aromatic Acids and Their Homologues. International Journal of Molecular Sciences 2017, 18 (5), 1074. doi:10.3390/ijms18051074

Chambers, C. S.; Holečková, V.; Petrásková, L.; Biedermann, D.; Valentová, K.; Buchta, M.; Křen, V., The silymarin composition… and why does it matter??? Food Research International 2017, 100 (Part 3), 339-353.

Valentová, K.; Káňová, K.; Di Meo, F.; Pelantová, H.; Chambers, C.; Rydlová, L.; Petrásková, L.; Křenková, A.; Cvačka, J.; Trouillas, P.; Křen, V., Chemoenzymatic preparation and biophysical properties of sulfated quercetin metabolites. Int. J. Mol. Sci. 2017, 18 (11), 2231. doi: 10.3390/ijms18112231

Catapano, M.; Tvrdý, V.; Karlíčková, J.; Migkos, T.; Valentová, K.; Křen, V.; Mladěnka, P., The Stoichiometry of Isoquercitrin Complex with Iron or Copper Is Highly Dependent on Experimental Conditions. Nutrients, 2017, 9 (11), 1193.  doi: 10.3390/nu9111193

Ajdžanović V, Jarić I, Miler M, Filipović B, Šošić-Jurjević B, Ristić N, Milenkovic D, Milošević V. Diosgenin-caused changes of the adrenal gland histological parameters in a rat model of the menopause. Acta Histochem. 2017 Jan;119(1):48-56. doi: 10.1016/j.acthis.2016.11.006.

Miler M, Jarić I, Živanović J, Ajdžanović V, Tanić N, Milošević V, Šošić-Jurjević B. Acta Histochem. Citrus flavanones mildly interfere with pituitary-thyroid axis in old-aged male rats. 2017 Apr;119(3):292-301. doi: 10.1016/j.acthis.2017.02.005.

Pedro Mena,Dolores González de Llano, Nicoletta Brindani,Adelaida Esteban-Fernández, Claudio Curti, María VictoriaMoreno-Arribas, Daniele Del Rio,Begoña Bartolomé. 5-(3′,4′-Dihydroxyphenyl)-γ-valerolactone and its sulphate conjugates, representative circulating metabolites of flavan-3-ols, exhibit anti-adhesive activity against uropathogenic Escherichia coli in bladder epithelial cells. Journal of Functional Foods, 29, 2017, Pages 275-280.

Cueva C, Gil-Sánchez I, Ayuda-Durán B, González-Manzano S, González-Paramás AM, Santos-Buelga C, Bartolomé B, Moreno-Arribas MV. An Integrated View of the Effects of Wine Polyphenols and their Relevant Metabolites on Gut and Host Health. Molecules. 2017 Jan 6;22(1). pii: E99. doi: 10.3390/molecules22010099.

Esteban-Fernández A, Rendeiro C, Spencer JP, Del Coso DG, de Llano MD, Bartolomé B, Moreno-Arribas MV. Neuroprotective Effects of Selected Microbial-Derived Phenolic Metabolites and Aroma Compounds from Wine in Human SH-SY5Y Neuroblastoma Cells and Their Putative Mechanisms of Action. Front Nutr. 2017;4:3. doi: 10.3389/fnut.2017.00003.

S. Moco, D. Barron (2017) Bioactive interactions in food and natural extracts. In: Nutrigenomics and Proteomics in Health and Disease, ed. by M. Kussmann and P. Stover, Wiley, 67-91. DOI: 10.1002/9781119101277.

P. Coulerie, Y. Ratinaud, S. Moco, L. Merminod, M. Naranjo Pinta, J. Boccard, L. Bultot, M. Deak, K. Sakamoto, E. Ferreira Queiroz, J.-L. Wolfender, D. Barron (2016) Standardized LC×LC-ELSD fractionation procedure for the identification of minor bioactives via the enzymatic screening of natural extracts, Journal of Natural Products, 79(11): 2856-64. DOI:10.1021/acs.jnatprod.6b00628.

Dietary Polyphenols against Metabolic Disorders: How Far Have We Progressed in the Understanding of the Molecular Mechanisms of Action of These Compounds?García-Conesa MT1. Crit Rev Food Sci Nutr. 2017 Jun 13;57(9):1769-1786. doi: 10.1080/10408398.2014.980499.

Gene expression changes in colon tissues from colorectal cancer patients following the intake of an ellagitannin-containing pomegranate extract: a randomized clinical trial. María A. Nuñez-Sánchez, Antonio González-Sarrías, Rocío García-Villalba, Tamara Monedero-Saiz, b, Noelia V. García-Talavera, María B. Gómez-Sánchez, Carmen Sánchez-Álvarez, Ana M. García-Albert, Francisco J. Rodríguez-Gil, Miguel Ruiz-Marín, Francisco A. Pastor-Quirante, Francisco Martínez-Díaz, Francisco A. Tomás-Barberán, Juan Carlos Espín, María-Teresa García-Conesa. Journal of Nutritionnal Biochemistry 2017, 42: 126-133 Abstract

Comprehensive characterization of the effects of ellagic acid and urolithins on colorectal cancer and key-associated molecular hallmarks: MicroRNA cell specific induction of CDKN1A (p21) as a common mechanism involved. Antonio González-Sarrías, María Angeles Núñez-Sánchez, Joao Tome-Carneiro, Francisco A. Tomás-Barberán, María Teresa García-Conesa and Juan Carlos Espín, Mol. Nutr. Food Res.2016, 60, 701–716. doi: 10.1002/mnfr.201500780

In vivo relevant mixed urolithins and ellagic acid inhibit phenotypic and molecular colon cancer stem cell features: A new potentiality for ellagitannin metabolites against cancer. María-Angeles Núñez-Sánchez, Ankur Karmokar, Antonio González-Sarrías, Rocío García-Villalba, Francisco A. Tomás-Barberán, María Teresa García-Conesa, Karen Brown, Juan Carlos Espín, Food and Chemical Toxicology, 92 (2016) 8e16. doi: 10.1016/j.fct.2016.03.011

MicroRNAs expression in normal and malignant colon tissues as biomarkers of colorectal cancer and in response to pomegranate extracts consumption: Critical issues to discern between modulatory effects and potential artefacts. Nuñez-Sánchez MA, Dávalos A, González-Sarrías A, Casas-Agustench P, Visioli F, Monedero-Saiz T, García-Talavera NV, Gómez-Sánchez MB,Sánchez-Álvarez C, García-Albert AM, Rodríguez-Gil FJ, Ruiz-Marín M, Pastor-Quirante FA, Martínez-Díaz F, Tomás-Barberán FA, García-Conesa MT, Espín JC. Mol Nutr Food Res. 2015 Oct; 59(10):1973-86. doi: 10.1002/mnfr.201500357

Association between Both Total Baseline Urinary and Dietary Polyphenols and Substantial Physical Performance Decline Risk in Older Adults: A 9-year Follow-up of the InCHIANTI Study. Rabassa M, Zamora-Ros R, Andres-Lacueva C, Urpi-Sarda M, Bandinelli S, Ferrucci L, Cherubini A. J Nutr Health Aging.2016; 20(5):478-85. doi: 10.1007/s12603-015-0600-2

Association of habitual dietary resveratrol exposure with the development of frailty in older age: the Invecchiare in Chianti study. Rabassa M, Zamora-Ros R, Urpi-Sarda M, Bandinelli S, Ferrucci L, Andres-Lacueva C, Cherubini A. Am J Clin Nutr.2015; 102(6):1534-42. doi: 10.3945/ajcn.115.118976



This article has been corrected to indicate the right COST Action number of POSITIVe


Inhibitory effects of chickpea and Tribulus terrestris on lipase, α-amylase and α-glucosidase. Ercan P, El SN. Food Chem.2016; 205:163-9. doi: 10.1016/j.foodchem.2016.03.012

Corrigendum to "Inhibitory effects of chickpea and Tribulus terrestris on lipase, α-amylase and α-glucosidase" [Food Chem. 205 (2016) 163-169]. Ercan P, El SN. Food Chem. 2016 Oct 1;208:338. doi: 10.1016/j.foodchem.2016.04.062