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Microgenomics International Symposium 2014

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Thursday, May 15 2014

8:15-9:20

 

Registration

 

 

9:20-9:30

 

Welcome / Introduction (organizer)

 

 

Session 1: Sampling methods (flow cytometry, laser capture microdissection, DEP array, Single cell picking ...)

Chariman: Marc Dalod

9:30-10:15

Keynote lecturer

Characterization of very rare immune cell types through their purification and gene expression profiling : different tools and strategies to obtain pure or enriched cell populations,  advantages and disadvantages of each tool and their complementarily)

Marc Dalod, CNRS-Centre d'immunologie de Marseilles-Luminy France

10:15-10:45

Invited lecturer

DeCIPHEr critical steps in a flow cytometry experiment to ensure reproducible and high quality microgenomic datatsets

Hervé Luche, Immuno-Phenotyping Module, Inserm, France

Click to download presentation

10:45-11:15

 

Coffee break-sponsors

 

 

11:15-11:45

Invited lecturer

Correlating function and gene expression at the level of individual dopamine neurons - form mice to men

Birgit Liss, Ulm University, Germany

 Not available

11:45-11:55

 

Microgenomics and Epistemology

Thomas Heams, France

 

11:55-12:05

 

Cell encapsulation for multiplex culture on a chip

Ophelie Berthuy, France

 Not available

 

 

 

 

 

Session 2: Microgenomics and DNA

Chairman: Christoph Klein

Chariman: Christoph Klein

12:05-12:50

Keynote lecturer

Molecular profiling of single circulating tumor cells: Impact for therapy selection and the understanding of cancer evolution

Christoph Klein, Universität Regensburg, Germany

 Not available

12:50-13:00

 

Are aptototic circulating tumor cells (CTC) suitable for molecular analysis?

Elisabetta Rossi, Italy

 

13:00-14:30

 

Lunch + posters + sponsors

 

 

14:30-15:00

 

Circulating tumor cells: from enrichment to single cell sequencing

Pamela Pinzani,University of Florence, Italy

click to download Presentation

15:00-15:10

 

Single cell genetic analysis helps validating cytopathological identification of CTC in patients with Clear Cells Renal Carcinoma

Patrizia Paterlini-Brechot,France

 

 

 

 

 

 

Session 3a: Microgenomics and RNA1 : MIQE guideline and microgenomics

Chairman:Mikael Kubista

Chariman: Mikael Kubista

15:10-15:55

Keynote lecturer

Taking Expression Profiling to New Dimensions

Mikael Kubista,  TATAA Biocenter, Institute of Biotechnology AS CR in Prague, Czech Republic

click to download Presentation

15:55-16:05

 

Droplet microfluidics for gene expression study at the single cell level

Davide Ferraro,France

 Click to download presentation

16:05-16:35

 

Coffee break-posters

 

 

16:35-17:05

Invited lecturer

Driving Genomics to the Single-Cell Level:  Analysis of RNA Expression Using qPCR and RNAseq

Ken Livak,Fellow Fluidigm Corporation South San Francisco, USA

click to download Presentation

17:05-17:35

Invited lecturer

Gene expression profiling from Single Laser Capture Microdissected (LCM) Cells 

Stephen Jackson, PhD,Life Sciences Solutions, Thermo Fisher Scientific,South San Francisco, USA

 

17:35-17:45

 

Single-cell transcriptional analysis of FACS-sorted kidney macrophages

Julie Bellière,France

 Not available

17:45-17:55

 

Gene expression of autoreactive T cells - Biomarker and pathogenesis of Type 1 diabetes

Anne Eugster,Germany

click to download Presentation

17:55-19:00

 

Poster session + French Wine & Cheese

 

 

20:00-22:30

 

Dinner cruise on the Seine

 

 

Friday, May 16 2014

 

 

 

 

 

Session 3b: Microgenomics and microRNA and NGS

Chairman:Michael W. Pfaffl

Chariman: Michael W. Pfaffl

9:00-9:45

Keynote lecturer

Exosome isolation and holistic expression profiling using RNA-Seq and RT-qPCR

Michael W. Pfaffl,Technical University of Münich, Germany

9:45-10:15

Invited lecturer

Measuring microRNA expression in size-limited samples

Kai Peter Höfig, Munich University, Germany

click to download Presentation

10:15-10:25

 

Towards a comprehensive single cell expression profiling

Herbert Auer, Spain

 Not available

10:25-10:35

 

A combination of microgenomics approaches to understand the ipmpact of gut microbiota on the regulation of the hypothalamo-pituitary-adrenal axis in rats

Bénédicte Langellier, France

 

10:35-11:15

 

Coffee break - posters

 

 

11:15-11:45

Invited lecturer

Comparative analysis of RNA sequencing methods for degraded or low-input samples

Rahul Satija, Broad Institute of MIT, Massachusetts, USA 

click to download Presentation

11:45-11:55

 

Low RNAseq microdissected plant tissue

Bernard Dubreucq, France

 

11:55-12:10

 

GOLD SPONSOR presentation: "Genomic Characterization of Challenging or Limited Research Samples using the Ion AmpliSeq™ Targeted Re-Sequencing Technology"

Dr. Stephen Jackson, Life Technologies/ Life Technologies/Thermo Fischer Scientific, USA

 

12:10-14:00

 

Lunch 

 

 

 

 

 

 

 

Session 4: Microgrenomics and Proteins

Chairman:Lance Liotta 

14:00-14:45

Keynote lecturer

Advantages or disadvantages of different methods for proteomics analysis 

Lance Liotta, George Mason University, USA

 

14:45-14:55

 

Oocytomics: combining transcriptomics and proteomics to understand post-transcriptional regulation in bovine oocytes

Rozenn Dalbies-Tran, France

Not available

14:55-15:05

 

Combining surface plasmon resonance and mass spectrometry to identify Bone Morphogenetic Protein (BMP) interactants

Catherine Taragnat, France

 

15:05-15:35

 

Coffee break - Posters

 

 

15:35-16:05

Invited lecturer

Mass Spectrometry Imaging coupled to Microproteomics: From  Imaging to identification of proteins on tissue section

Maxence Wisztorski, University of Lille I, France

click to download Presentation

16:05-16:35

Invited lecturer

Unlocking the prognostic significance of microdissected proximal lesions from human colon

Daniel W. Rosenberg, University of Connecticut Health Center USA

click to download Presentation

16:35-16:45

 

Closing remarks