Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu INRA Logo institutions

SPS - Saclay Plant Sciences

Adaptation to Nitrogen Availability in Arabidopsis and Brachypodium (AdaptN)

Coordinator: Anne Krapp

PhD project selected in 2012 – Person recruited: Camille Chardin

Nitrogen is one of the most important macronutrients for plants. In the soils, nitrogen exists under different organic and inorganic forms. Plants preferentially use nitrate, which is often in limiting amount in soils. Recently, it was shown that the NLP7 transcription factor is a master regulator of the primary nitrate response and that the NPF6.3 nitrate transporter plays a role in the nitrate signal sensing. By studying the simple and double mutant’s biomass and sentinel gene regulation in response to nitrate, we observed additive phenotypes. In addition we showed that the nuclear relocation mechanism of NLP7 in response to nitrate is still active in the npf6.3 mutant background. All together, these results showed that NLP7 and NPF6.3 are not in the same signalling pathway. Only a few regulators of the nitrate response are known. Interestingly the direct target genes of NLP7 are highly enriched for regulatory proteins such as other transcription factors or protein kinases, which places NLP7 at a high hierarchical place in the nitrate signalling pathway. In order to identify molecular events downstream of NLP7, we studies two NLP7 direct targets. In protoplasts, we have shown that MAPKKKK13/14 are able to activate Group C MAPKs via MKK3. In addition we showed in planta that nitrate addition indeed triggered the activation of group C MAPKs and that this activation is dependent on NLP7 and MKK3. Furthermore we obtained first indications that nitrate-dependent developmental traits are partially impaired in the single mutants mapkkk13 and mapkkk14, and the double mutant mpkkk13mpkkk14.

Publications:

Chardin C., Girin T., Roudier F., Meyer C., Krapp A. (2014). The plant RWP-RK transcription factors: key regulators of nitrogen responses and of gametophyte development. Journal of Experimental Botany 65(19): 5577-5587.

Chardin C., Schenk S.T., Hirt H., Colcombet J., Krapp A. (2017). Review: Mitogen-Activated Protein Kinases in nutritional signaling in Arabidopsis. Plant Sci 260: 101-108.

Girin T., David L.C., Chardin C., Sibout R., Krapp A., Ferrario-Mery S., Daniel-Vedele F. (2014). Brachypodium: a promising hub between model species and cereals. Journal of Experimental Botany 65(19): 5683-5696.

Krapp A., David L.C., Chardin C., Girin T., Marmagne A., Leprince A.-S., Chaillou S., Ferrario-Mery S., Meyer C., Daniel-Vedele F. (2014). Nitrate transport and signalling in Arabidopsis. Journal of Experimental Botany 65(3): 789-798.

Communications in conferences:

Camille Chardin - PhD student retreats

Anne Krapp - Plenary lecture at Nitrogen 2016, Montpellier