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INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

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SPS - Saclay Plant Sciences

Arabidopsis Interactome Mapping – Developpement of a SPS platform with identification of pairs by HTS (INTERACT)

Coordinator: Claire Lurin

Post-doc project selected in 2013 – Person recruited: Damien Guillaumot

Protein-protein interactions are a critical element of biological systems and the analysis of interaction partners can provide valuable hints about unknown functions of a protein. To date, the yeast 2 hybrid system is the only method to probe protein-protein interaction routinely in high-throughput. Between 2006 and 2011, the “Organellar Gene Expression” team was involved in an  international project of Arabidopsis Interactome Mapping (AIM project). A set of c. 12,000 Arabidopsis genes were used to construct a high-coverage network. This network covers about one fifth of the whole Arabidopsis network and needs to be further completed. During the AIM project, all the Y2H experiments were done in Boston; the objectives of the subsequent SPS INTERACT project were to become autonomous in Y2H screening at IPS2 and to develop a SPS tool available for the Arabidopsis community.

This INTERACT project involved 4 SPS teams and demonstrated the efficiency and utility of such a platform for the SPS laboratories, in particular, and the Arabidopsis community, in general. During the pilot experiment, we  screened the prey AtORFeome library with about 100 bait proteins. By improving of the protocol which was previously used for the AIM project, we  developed and validated an Y2H high-throughput liquid pipeline. In 2016, the InterATOME plateform was settled up and opened.

Publications:

Guillaumot D., Lopez-Obando M., Baudry K., Avon A., Rigaill G., Falcon de Longevialle A., Broche B., Takenaka M., Berthomé R., De Jaeger G., Delannoy E., Lurin C. (2017). Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria. Proceedings of the National Academy of Sciences of the United States of America In press.