Know more

Our use of cookies

Cookies are a set of data stored on a user’s device when the user browses a web site. The data is in a file containing an ID number, the name of the server which deposited it and, in some cases, an expiry date. We use cookies to record information about your visit, language of preference, and other parameters on the site in order to optimise your next visit and make the site even more useful to you.

To improve your experience, we use cookies to store certain browsing information and provide secure navigation, and to collect statistics with a view to improve the site’s features. For a complete list of the cookies we use, download “Ghostery”, a free plug-in for browsers which can detect, and, in some cases, block cookies.

Ghostery is available here for free: https://www.ghostery.com/fr/products/

You can also visit the CNIL web site for instructions on how to configure your browser to manage cookie storage on your device.

In the case of third-party advertising cookies, you can also visit the following site: http://www.youronlinechoices.com/fr/controler-ses-cookies/, offered by digital advertising professionals within the European Digital Advertising Alliance (EDAA). From the site, you can deny or accept the cookies used by advertising professionals who are members.

It is also possible to block certain third-party cookies directly via publishers:

Cookie type

Means of blocking

Analytical and performance cookies

Realytics
Google Analytics
Spoteffects
Optimizely

Targeted advertising cookies

DoubleClick
Mediarithmics

The following types of cookies may be used on our websites:

Mandatory cookies

Functional cookies

Social media and advertising cookies

These cookies are needed to ensure the proper functioning of the site and cannot be disabled. They help ensure a secure connection and the basic availability of our website.

These cookies allow us to analyse site use in order to measure and optimise performance. They allow us to store your sign-in information and display the different components of our website in a more coherent way.

These cookies are used by advertising agencies such as Google and by social media sites such as LinkedIn and Facebook. Among other things, they allow pages to be shared on social media, the posting of comments, and the publication (on our site or elsewhere) of ads that reflect your centres of interest.

Our EZPublish content management system (CMS) uses CAS and PHP session cookies and the New Relic cookie for monitoring purposes (IP, response times).

These cookies are deleted at the end of the browsing session (when you log off or close your browser window)

Our EZPublish content management system (CMS) uses the XiTi cookie to measure traffic. Our service provider is AT Internet. This company stores data (IPs, date and time of access, length of the visit and pages viewed) for six months.

Our EZPublish content management system (CMS) does not use this type of cookie.

For more information about the cookies we use, contact INRA’s Data Protection Officer by email at cil-dpo@inra.fr or by post at:

INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu INRA Logo institutions

SPS - Saclay Plant Sciences

Intracellular anion dynamics (IntraDyn)

Coordinator: Alexis De Angeli

PhD project selected in 2014 – Person recruited: Elsa Demes-Causse

Ion transport across biological membranes is a fundamental process of every living cell that is involved in, for example, nutrition, signalling and in generating membrane potentials. The transport of solutes across membrane is controlled by specialised membrane proteins named transporters. Transporters are divided in two active and passive transporters, depending on the energetic of the process they catalyse. The separation between active and passive transporters became blurry after the discovery that within the same protein family, the CLC (Chloride Channel) family, different members were either active either passive transporters. This finding raised several questions about the structural determinants separating the two transporters types. In the model plant Arabidopsis thaliana there are seven members of the CLC family localised in intracellular membranes. The A. thaliana CLCs are involved in important physiological processes like nitrate storage, abiotic stress tolerance and stomata movements generating anion fluxes across intracellular membranes.  Despite their importance the functional characteristics of the majority of the Arabidopsis CLCs are unidentified. Moreover, in plant cells the dynamic of intracellular anion fluxes during cellular processes are largely unknown despite their fundamental role in in plant nutrition, salt stress tolerance and the regulation of stomata aperture. The present project  has two aims: i) characterising the functional characteristics of A. thaliana CLCs using an electrophysiological approach; ii) understanding the dynamic of intracellular anion fluxes in Arabidopsis cells using biosensors. The combination of the results will be a major step in understanding intracellular anion physiology.

Publications:

Eisenach C., De Angeli A. (2017). Ion Transport at the Vacuole during Stomatal Movements. Plant Physiology 174(2): 520-530.

Communications in conferences:

- EMBO/FEBS Lecture"Ion channels and Transporters" Course 2017, Erice Italy  (Alexis De Angeli)

- EPSR, Barcelona Spain (Elsa DEMES)

- 6th Biosensor meeting, Orsay France, Alexis De angeli  (2016)